Journal: Mediators of Inflammation

Article Title: Adipose Tissue-Specific Deletion of 12/15-Lipoxygenase Protects Mice from the Consequences of a High-Fat Diet

doi: 10.1155/2012/851798

Figure Lengend Snippet: Primer sequences used in SYBR-based real-time RT-PCR amplification of mouse cDNA.

Article Snippet: Insulin and high-molecular-weight adiponectin were measured in serum by ELISA (Mercodia, Uppsala, Sweden and ALPCO Diagnostics, Salem, NH, USA, resp.).

Techniques: Quantitative RT-PCR, Amplification

Journal: Mediators of Inflammation

Article Title: Adipose Tissue-Specific Deletion of 12/15-Lipoxygenase Protects Mice from the Consequences of a High-Fat Diet

doi: 10.1155/2012/851798

Figure Lengend Snippet: Fat-specific 12/15-LO-deficient mice exhibit decreased inflammation in adipose tissue following a high-fat diet regimen. (a) Epididymal adipose tissue weight and adipocyte size were measured in mice after the 16-week feeding regimen. Hematoxylin and eosin stained sections of epididymal adipose tissue are shown. Scale bar = 50 μ M. (b) mRNA measurements of key proinflammatory and anti-inflammatory genes were examined by RT-PCR in epididymal adipose tissue from mice. (c) Adiponectin mRNA and high-molecular-weight (HMW) adiponectin protein were measured in isolated epididymal adipocytes and serum by RT-PCR and ELISA, respectively. (d) Protein measurement of collagen 6 (Col6) was performed from isolated epididymal adipocytes and quantified. Representative western blots are shown, and separate panels for each antibody represent the same exposure from the same gel. All mRNA and protein data were normalized to total actin, and the fold changes in expression were calculated relative to control. All data represent the means ± SEM; n = 3–6. * P < 0.05, † P < 0.02, ‡ P < 0.001 versus control, unless otherwise indicated. Chow: chow diet; HFD: high-fat diet; wt: wild type 12/15 - LO loxP / loxP .

Article Snippet: Insulin and high-molecular-weight adiponectin were measured in serum by ELISA (Mercodia, Uppsala, Sweden and ALPCO Diagnostics, Salem, NH, USA, resp.).

Techniques: Staining, Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Isolation, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing